Malignant Melanoma is the most lethal of the
skin cancers, and histological diagnosis
is highly subjective and difficult, especially when biopsied by the shave
technique. The diagnosis is further
complicated by the need to differentiate Malignant Melanoma from other melanocytic mimickers such as Spitz Nevus.
Acupath’s Melanoma and Spitz Nevus FISH Panels are used as an adjunct to
conventional H&E and IHC studies, particularly in ambiguous cases, to identify genetic abnormalities
that are confirmed to be specific to Malignant Melanoma and Spitz Nevus. Diagnosis by the FISH technique can be
performed on the same formalin-fixed, paraffin-embedded tissue used to make H&E slides.
Validations of AcuProbe
Melanoma and AcuProbe
Spitz
have been approved by the New York State
Department of Health (NYSDOH).
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A Powerful, Objective and Rapid Diagnostic Tool in |
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Malignant Melanoma Diagnosis |
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- Expand your services by introducing AcuProbe™ to your case workups.
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- Confirm or exclude the histological diagnosis of Malignant
Melanoma and differentiate Malignant Melanoma from other melanocytic simulators:
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• Atypical Dysplastic
Nevus |
• Spitz Nevus |
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• Persistent Nevus |
• Desmoplastic Spitz Nevus |
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- Diminish the amount of 2nd opinions by confirming your diagnosis,
and lessen your risk of malpractice.
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- Remain a leader in the
industry by utilizing the latest testing advancement in dermatopathology.
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- Use for your research studies or
clinical trial studies.
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Objective, Quantifiable Confirmation by
FISH |
AcuProbe Melanoma: Acupath’s molecular pathologists have validated four specific
genetic FISH (fluorescence in situ hybridization) probes that can detect
relative gains and losses of genetic mutations which can differentiate
Malignant Melanoma from benign melanocytic lesions. These
probes were developed based on previously performed studies. The genes of interest are RREB1
(6p25 gain), MYB (6q23 loss), CCND1 (11q13 gain). CEP6 is used as an internal control (Abbott Molecular).
AcuProbe Spitz: An additional probe, HRAS (11p15) which is used in conjunction with CCND1 (used as an internal control), is unique to Acupath and has been observed in Spitz Nevi, especially Desmoplastic Spitz Nevi.
AcuProbe Melanoma Comprehensive: Acupath dermatopathologists choose all IHC, special (billable)
stains and FISH testing for diagnosis under consideration (Melanoma or Spitz Nevus)
or as deemed
medically necessary, unless otherwise indicated by ordering physician in the Test Request section on the requisition.
Acupath’s Melanoma and Spitz Nevus FISH probes are original
designs and have been specifically developed to optimize the area of coverage
allowing for an increased specificity and sensitivity.
References
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How to submit a specimen |
The AcuProbe FISH test is useful for melanocytic neoplasms only, specifically Malignant Melanoma and Spitz Nevus. Acupath will only perform the FISH test if the physician has indicated a clinical diagnosis of Melanoma or Spitz Nevus on the requisition.
PHYSICIANS
For new biopsies:
- Submit tissue in
formalin container as you would for routine histology. No
additional specimen is required.
To request FISH testing on cases previously
diagnosed by another lab:
- Please
contact the lab that diagnosed the case(s) and request shipment of the items
below to Acupath.
- Fax an Acupath requisition or
letter (on your letterhead) to Acupath at 516-977-5396 or 516-977-6793 requesting
the FISH test and include the patient(s) name(s) and the name of the lab who is
sending us the items.
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• Paraffin block or 3-5 |
• H&E slide with the area of interest circled |
• Pathology report with available patient history/clinical information |
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unstained slides |
• All other
representative IHC slides |
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LABORATORIES
For histology and FISH:
- Submit tissue in
formalin container as you would for routine histology. No
additional specimen is required.
For FISH only:
- Please
fill out an Acupath requisition and ship
the items below to Acupath:
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• Paraffin block or 3-5 |
• H&E slide with the area of interest circled |
• Pathology report with available patient history/clinical information |
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unstained slides |
• All other
representative IHC slides |
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